Fig. S9.

Relative forebrain expression of APP, Aβ, αSyn, and synapsins in 3-mo-old mice used in the study. (A) Western blot (WB) analyses of human APP and total Aβ in forebrains of young WT, J20, TgI2.2, and J20×TgI2.2 mice using 6E10. Actin was used as internal loading control. (B) Quantification of the relative protein levels for APP and Aβ. (Histograms represent the mean ± SD; Student t test with Bonferroni correction, ^★P < 0.05 vs. J20, n = 6 group per genotype.) (C) Representative confocal images of CA1 hippocampal neurons immunostained for the MAP2 (blue channel), αSyn (4D6; green), and synapsins (magenta) revealed no aberrant formation of LB inclusions in 3-mo-old TgI2.2 and J20×TgI2.2 mice. A subtle global reduction in the signal for SYN could also be noticed (n = 6 sections per animals; n = 3–6 animals per genotype). (Scale bars: 20 μm.) M, months.