Primary blasts of AML patients (n = 4) were plated in triplicate in methylcellulose medium supplemented with rapamycin (E7+RAP; 10 nM), rhEGFL7 (250 nM) alone, or in combination. Colonies with more than 50 cells were enumerated after 10 d of culture, *P < 0.05, **P < 0.01, and ***P < 0.001 vs. CTRL; #P < 0.05, ##P < 0.01, ###P < 0.001, rEGFL7 vs. E7+RAP. There was no statistical difference between rapamycin and E7+RAP in all tested patient samples. CTRL, control; E7, rEGFL7; RAP, rapamycin.