Skip to main content
. 2017 May 22;114(23):6116–6121. doi: 10.1073/pnas.1619424114

Fig. 5.

Fig. 5.

Spatiotemporal dissemination of the immune cell infiltration and BSCB opening do not follow the pattern of P-selectin vascular activation in the spinal cord of PLP-induced EAE mice. (A) Immunohistological images of the infiltrated areas of the PLP-induced EAE spinal cord. CD4+ leukocyte (green), fibrinogen (red), and cell nuclei (blue) show the spatiotemporal dissemination of the immune cell infiltration. Each line of images corresponds to a spinal cord section (in order from top to bottom: cervical, thoracic, lumbar, and sacral). Each column corresponds to a different stage of the disease (sham, first relapse, recovery, and second relapse; n = 3 per group). (B) Corresponding quantification. ND, nondetected; NS, not significant; *P < 0.05 vs. sham animals in the same structure; #P < 0.05 vs. indicated condition (n = 3 mice for each clinical group). (C) Immunohistological image of whole section of PLP-induced EAE spinal cord (peak of the first relapse) with a low magnification (Left) and a high magnification (Right). [Scale bars: 500 µm (Left) and 100 µm (Right).] GM, gray matter; WM, white matter. (D) Quantification of the distribution of the CD4+ T-cell infiltration between the white matter (white bar) and in the gray matter (gray bar). #P < 0.05. (E) Spinal cord photographs after i.v. Evans blue dye injection in PLP-induced EAE. (F) Ex vivo optical imaging of Evans blue fluorescence. (G) Quantification of fluorescence in spinal cord tissues. *P < 0.05; **P < 0.01; ***P < 0.001 vs. the same structure in sham animals; #P < 0.05, ##P < 0.01 vs. indicated condition. See Table S1 for detailed sample size.