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Although 5 μM SKF36365 (SKF) does not affect Orai1-based SOCE (Fig. 1G), higher concentrations do. The figure shows SOCE evoked by thapsigargin (Tg) in HEK293 cells transfected with inhibitory amounts of Orai1 (1 μg plasmid per 60-mm dish) and an SOCE-rescuing amount of STIM1 (1 μg plasmid per 60-mm dish) in the absence or presence of 25 μM or 100 μM SKF96365.
