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. 2017 May 22;114(23):E4631–E4640. doi: 10.1073/pnas.1703178114

Fig. 4.

Fig. 4.

SLC22A3 knockdown increases normal esophageal cell motility. (A) The percentage of edited SLC22A3 transcripts was determined by pyrosequencing in normal esophageal NE1cells. (B and C) Expression of SLC22A3 in shSLC22A3 stably transfected NE1 clones (NE1-shSLC22A3-1 and -2) was confirmed by qPCR (B) and Western blotting (C). Nontemplate shRNA-transfected cells (NE1-NTC) were used as controls. **P < 0.001, ANOVA with post hoc test. (D) Relative growth rates of SLC22A3-repressed NE1 cells were compared with control cells by XTT assay. The results are expressed as the mean ± SD of at least three independent experiments. P > 0.05; ANOVA. (E) The effect of depleted SLC22A3 on cell migration was determined using the wound-healing assay. Representative images show that SLC22A3-repressed NE1 cells migrated faster along the wound edge than the control cells. (F) The representative images (Left and Upper Right) and quantification of cells (Lower Right) from the indicated NE1 cells that invaded through Matrigel-coated membrane. Results are shown as mean ± SD of three independent experiments. (Scale bars: 200 μm.) **P < 0.001, ANOVA with post hoc test.