Fig. 1.

Transient removal of Pax6 proteins reveals their roles in the development of all head structures derived from the eye-antenna disc. (A) Wild-type adult head. (B) DE-GAL4 > toy RNAi + ey RNAi flies lack all head structures derived from the eye-antennal discs (100%, n = 30). (C) In wild-type larvae, eye-antennal discs (green arrows) are found between the mouth hooks and brain. (D) Eye-antennal discs are absent in DE > toy RNAi + ey RNAi flies (red arrows, 100%, n = 27). (E–R) Flies are of the following genotype: tub-GAL80^ts; DE-GAL4 > toy RNAi + ey RNAi. (E) Quantification of phenotypes observed when animals are transferred from 18 °C to 30 °C to remove Ey and Toy (n = 45, 50, 30, 38, 48, and 28, respectively). x axis = hours AEL at 18 °C. y axis = phenotype percent. (F–K) Light-microscope images of adult heads. Embryos/larvae were kept at 18 °C AEL for 0, 24, 48, 72, 96, and 120 h before being transferred to 30 °C. (L–Q) Corresponding third-instar eye-antennal discs of larvae from F–K. (R) Quantification of phenotypes observed when animals are transferred from 30 °C to 18 °C to restore Ey and Toy (n = 57, 53, 55, 55, and 50, respectively). x axis = hours AEL at 30 °C; y axis = phenotype percent. (Scale bars, 100 µm.)