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ey continues to be expressed in the eye-antennal discs of toy1-null mutants. (A) Schematic depicting the deletion of the toy locus using CRISPR. We are calling the resulting mutant toy1. (B–E) Third-instar eye-antennal discs from wild-type flies (B) and toy1-null mutants (C–E) stained with Ey (green) and F-actin (purple). Anterior is to the right. (F–I) Adult heads from toy1-null mutants. (Scale bars, 100 µm.)
