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. 2017 May 23;114(23):E4658–E4665. doi: 10.1073/pnas.1614596114

Fig. 1.

Fig. 1.

The GLB-5 neuroglobin broadens C. elegans’s O2 preference. (A) Aerotaxis behavior. Distribution of animals in a 0–21% O2 gradient. n = 6. Plots show mean ± SEM. (B) The glb-5(Haw) allele broadens C. elegans’s O2 preference. High O2 avoidance index = (fraction of animals in 7–14% O2) − (fraction of animals in 14–21% O2)/(fraction of animals in 7–21% O2). O2 preference (7–10% O2) = (fraction of animals in 7–10% O2)/(fraction of animals in 7– 21% O2). **P < 0.01, Mann–Whitney u test. Data are from A. (C) Mean Ca2+ responses of URX neurons to the indicated O2 ramp stimulus. n = 9–12. Shading represents SEM. The Ca2+ sensor is GCaMP6s coexpressed with mCherry (URX). Also shown are the responses of gcy-35; glb-5(tm5440) mutants; GCY-35 is required for measurable O2-evoked Ca2+ responses. The color bars represent the average response for each genotype at time point indicated by the black bar. Data show mean ± SEM. **P < 0.01, ANOVA with Tukey’s post hoc test. The O2 ramp stimulus (Top) shows the mean of four measurements ± SEM.