Fig. S8.

Cell density estimation and cell detection parameters. (A) Correlation between individual pixel fluorescence intensity in arbitrary units (A.U.) plotted against cell density estimated using a KDE at the same position as the pixel. Red line indicates the fitted line used to relate the two. (B) Fluorescence intensity cutoff for cell detection was chosen as the bottom of the elbow created when plotting number of cells detected vs. the intensity cutoff. Arrow indicates the cutoff chosen for use in segmentation.