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. 2017 Jun 13;3:17019. doi: 10.1038/celldisc.2017.19

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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UHRF1 knockdown in HBE cells results in a senescence-associated gene expression signature and global DNA hypomethylation. (a) Heat map of downregulated and upregulated genes using two independent shRNAs. (b, c) Most highly represented molecular pathways altered as a result of UHRF1 depletion based on gene ontology analysis. (d) HeLa cells were treated with 0, 0.1 or 1 μM 5-Aza for 1 week and genomic DNA was extracted. 5-methylated cytosine (5-MeC) levels were measured by DNA dot blot assay and total DNA was stained with methylene blue. (e) Genomic DNA was extracted from control and UHRF1 knockdown HBE cells, and the same procedure was used to determine the ratio of 5-MeC to total DNA as indicated for each condition.