Figure 6.

UHRF1 is prerequisite for the DNA replication factory formation. (a) Representative confocal images of control and UHRF1 knockdown cells co-stained with the indicated antibodies. Cells were collected 3 days after virus transduction when UHRF1 expression began to decrease. (b, c) Both UHRF1 and Ki67 expression in basal cells were examined at early time points after airway injury and quantification of cells with different UHRF1 and Ki67 expression levels were shown in c. These data indicate that 24-h post-injury was the time point when basal cells progressed to the S phase in the first cell cycle. Scale bar: 20 μm. (d, e) Representative confocal images of the tracheal epithelium from K5-CreER mice or Uhrf1^fl/fl;K5-CreER mice 24 h after SO₂ injury stained with the indicated antibodies. Top panels are projections generated from z-stack confocal images. Bottom panels are z-direction images. White arrowheads indicate cells without punctate PCNA staining patterns. Brown arrows indicate cells with punctate staining pattern in early to middle S phase. White arrows indicate cells with punctate staining pattern in late S phase. (f) Quantification of the percentages of UHRF1 status in punctate PCNA cells. Scale bar: 10 μm.