Fig. 4.

Effects of Wogonin on the renewal capacity of CD133⁺ CAL72 CSC. a The effect of Wogonin on inhibiting the number of spheres. b Single sphere formation of CD133⁺ CAL72 cells after 9 days in suspension culture containing Wogonin (0, 10, 20, 40, and 80 μM). c Wogonin reduced the clonogenicity of CD133⁺ CAL72 cells; each well was seeded with 1 × 10³ cells and incubated at 37 °C with 5% CO₂ after 24 h of Wogonin treatment. Then, the colonies were visualized by staining with 0.5% crystal violet. Data are represented as mean ± SEM (n = 3). ^∗ P < 0.05, ^∗∗ P < 0.01, ^**** P < 0.0001