Fig. 1.

Biomass accumulation of the WT, Δpdr-1, and pdr-1-comp strain. PEC pectin, ARN arabinan, AG arabinogalactan, PGA polygalacturonic acid, XLN xylan. Error bars represent standard deviation (n = 3). A The biomass of N. crassa WT, Δpdr-1, and pdr-1-comp was determined. Strains were grown for 3 days in 3 ml cultures with 1% polysaccharide as carbon source. Alternatively, the strains were pre-incubated for 16 h in medium containing 2% sucrose, then washed, transferred to a 2 mM monosaccharide medium and incubated for additional 3 days with regular medium switches. After incubation, the biomass was determined as dry weight. The samples were normalized to WT biomass. Significance was determined by an independent two-sample t-test of WT against Δpdr-1 or pdr-1-comp with *p < 0.05, **p < 0.01, and ***p < 0.001. B Same strains as mentioned under A were grown on either 2 mM d-GalA, 0.5 mM d-Xyl or 2 mM d-GalA and 0.5 mM d-Xyl. Significance was determined by an independent two-sample t-test of 0.5 mM d-Xyl against 2 mM d-GalA and 0.5 mM d-Xyl with **p < 0.01