Fig. 4.

Enzymatic activity assays and biomass determination of Δpdr-1 and complementation constructs. Strains were grown for 3 days on either 1% pectin or 1% xylan. A Supernatants from the WT, Δpdr-1, and pdr-1-comp strains were used to analyze pectate lyase activity. B endo-PGase activity of culture supernatants from the WT, Δpdr-1, and pdr-1-comp strains (n.d. stands for none detected). C Biomass of the strains by dry weight. Biomass of Δgh28-1 on 1% pectin was not measurable (n.m.), since supernatant was highly viscous and could not be separated from mycelium. Error bars represent standard deviation (n = 3). Significance was determined by an independent two-sample t-test of WT against all other strains with *p < 0.05, **p < 0.01, and ***p < 0.001