Figure 4.
Microglia number and morphologic status assessed by IBA-1 immunohistochemistry in E3FAD and E4FAD mice across dietary treatments. A, Representative images of microglial morphology associated with resting (type 1) and reactive (types 2 and 3) phenotypes. Scale bar, 40 µm. B–E, Densities (cells/mm2) of IBA-1-immunoreactive cells in E3FAD and E4FAD mice on control and Western diets were quantified in (B) entorhinal cortex, and hippocampal subregions (C) subiculum, (D) CA1, and E) CA2/3. F–I) Percentages of all IBA-1-immunoreactive cells scored as having reactive phenotype (types 2 and 3) were quantified in (F) entorhinal cortex, and hippocampal subregions (G) subiculum, (H) CA1, and (I) CA2/3. Data are presented as mean (±SEM) values; n = 7–11/group. E3FAD mice are shown as circles, E4FAD mice are shown as squares; control diet groups are indicated as open symbols, and Western diet groups as filled symbols. *, p < 0.05 relative to genotype-matched mice in control diet condition. #, p < 0.05 relative to E3FAD mice in same diet condition.