Fig. 2.

CYT997 induces apoptosis but not autophagy in prostate cancer cells. a DU145 and PC3 cells were treated with 60 nM CYT997 for the indicated times, and O₂ ^·− release was determined by electrochemical biosensor. b DU145 and PC3 cells were treated with the indicated concentrations of CYT997 for 8 h, and ROS generation was determined by DCFH-DA staining. c, d DU145 and PC3 cells were treated with the indicated concentrations of CYT997 for 24 h, and cell lysates were collected for Western blot with the indicated antibodies (c), and apoptosis was determined by Cell Death Detection Elisa kit (d). *p < 0.05; **p < 0.01