Fig. 4.

Dasatinib enhances in vitro cytotoxicity of CYT997 in prostate cancer cells. a DU145 and PC3 cells were treated with 60 nM CYT997 for 24 h, in the presence or absence of 50 nM dasatinib (Das). Cell lysates were collected for Western blot with the indicated antibodies. b–d PC3 cells were treated with 60 nM CYT997 for 24 h, in the presence or absence of 50 nM dasatinib. Cell death was determined by flow cytometry with Zombie Aqua staining (b), apoptosis was determined by Cell Death Detection Elisa kit (c), and cell invasion was determined by Matrigel-coated modified Boyden chambers (d). **p < 0.01