Figure 2.

BiFC for selected candidate XLG1 and 3 interacting proteins outside the nucleus. (A) At1g71030 (B) At1g44170 (C) At4g02380 (D) At2g38480 (E) At3g19640 (F) At5g66240. The XLGs were fused to the N-terminus of YFP and the prey proteins were fused to the C-terminus of YFP. The bait constructs nYFP-XLG1 and XLG3 are indicated at the top columns and the prey constructs are listed at the left rows. The transformation control is the mitochondria marker MT-RK. Each combination of the prey and bait has two panels; the upper one is the YFP signal indicated by complementation of cYFP and nYFP and the lower one is RFP indicating positive transformation. Bar = 50 μm