Table 2.
Important genes differentially expressed in THP-1 following 6 h treatments with LL-37
| Gene symbol | Fold changes WRT NT | Entrez ID | Function | |||
|---|---|---|---|---|---|---|
| CNT | LL37-20 | CNT–LL37 | CNT + LL37 | |||
| TSG101 | 2.1 | 1.0 | 6.8 | 1.0 | 7251 | Acts as a negative growth regulator |
| GRB2 | 1.5 | 1.0 | 5.2 | 1.0 | 2885 | Links cell surface GFRs and the Ras signaling pathway |
| IL9R | 1.0 | 2.6 | 2.6 | 2.5 | 3581 | Interleukin-3, 5 and GM-CSF signaling |
| MAP4K3 | 1.0 | 1.7 | 6.2 | 1.0 | 8491 | MAPK signaling pathway and TNF signaling |
| CFLAR | 1.0 | 1.0 | 6.3 | 1.0 | 8837 | Acts as an inhibitor of TNFRSF6 mediated apoptosis |
| ENPP1 | 3.0 | 1.0 | 7.0 | 1.0 | 5167 | Appears to modulate insulin sensitivity and function |
| RALBP1 | 1.0 | 3.1 | 4.5 | −2.6 | 10928 | Can catalyze transport of glutathione and xenobiotics |
| SUCLA2 | −1.8 | 4.0 | 5.6 | 4.8 | 8803 | Catalyzes succinyl-CoA production |
| ALOX5 | 1.0 | 1.0 | 6.0 | 1.0 | 240 | Catalyzes leukotriene biosynthesis and inflammation |
| MAOB | 1.0 | 4.6 | 4.3 | 1.0 | 4129 | Oxidative deamination of biogenic and xenobiotic amines |
| CCL20 | 5.5 | 9.2 | 10.1 | 1.0 | 6364 | Chemotactic to lymphocytes and neutrophils. Possesses antibacterial activity E. coli and S. aureus |
| IL33 | 1.0 | 1.0 | 3.5 | 1.0 | 90865 | Activates NF-kappa-B and MAPK signaling pathways |
| IL36G | 1.0 | 2.9 | 3.8 | 2.7 | 56300 | Activates NF-kappa-B and MAPK signaling pathways |
| SLC2A14 | 1.0 | 1.0 | 4.1 | 3.3 | 144195 | Facilitative glucose transporter |
| DEFB105B | 1.0 | 1.0 | 2.8 | 1.0 | 504180 | Has antibacterial activity |
| DEFA5 | 1.0 | 1.0 | 5.0 | 1.0 | 1670 | Antimicrobial activity against broad spectrum bacteria |
| EFR3A | −25.4 | 1.0 | 7.3 | 1.0 | 23167 | Signaling through PIP3K and G protein couples receptors |
| PLA1A | 1.0 | 6.2 | 5.2 | 1.0 | 51365 | Stimulate histamine production |
| INO80B | 1.0 | 1.0 | 2.8 | 1.0 | 83444 | Cell cycle arrests at the G1 phase of the cell cycle |
| TLE1 | 1.0 | 3.8 | 5.8 | 1.0 | 7088 | Inhibits NF-kappa-B-regulated gene expression |
| SKP2 | 1.0 | 2.3 | 7.1 | 1.0 | 6502 | involved in regulation of G1/S transition |
| NLRC4 | 1.0 | 1.0 | 2.9 | 1.0 | 58484 | Senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex |
| BTG3 | 1.0 | 5.7 | 6.1 | 1.0 | 10950 | Blocks cell cycle at G0/G1 to S phase |
| SLC22A15 | 5.6 | 1.0 | 9.5 | 9.0 | 55356 | Probably transports organic cations |
| IL11RA | 1.0 | 3.6 | 3.2 | 1.0 | 3590 | Involved in macrophage proliferation and differentiation |
| OGFOD2 | 1.0 | 4.9 | 6.9 | 5.4 | 79676 | Iron ion binding and oxidoreductase activity |
| IL17RE | 1.0 | 1.0 | 3.9 | 3.8 | 132014 | crucial regulator in innate immunity to bacterial pathogens |
| CCNE2 | 1.0 | 4.7 | 4.5 | 3.2 | 9134 | Blocks cell cycle at the G1-S phase |
| IRF1 | 1.0 | 1.0 | 4.7 | 1.0 | 3659 | Regulation of IFNs against viral and bacterial infections |