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Tobacco smoke constituents increase the fluorescence of the Ca2+-sensitive dye Fluo-4. HEK293T cells were cultured in 384-well plates and exposed to vehicle (− control), 1 μM thapsigargin (+ control), or 10 mM of the constituents as indicated. Data are shown as normalized peak changes in Fluo-4 emission (compared to vehicle). All n = 9/group. *p < 0.05.
