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The effects of tobacco smoke constituents on HEK293T cell viability. (A–C) HEK293T cells were cultured in 384-well plates for 24 hours, exposed to the chemicals of interest for 1 hour, and then stained with calcein-AM and propidium iodide to measure live and dead cells, respectively. Data are shown as the ratio calcein/propidium iodide emission (F495/F540). (D) Summary bar graph of data from (A–C) taken at 2.86 mM. All bars are n = 9 wells from three separate experiments. *p < 0.05.
