. Author manuscript; available in PMC: 2017 Jul 1.

Published in final edited form as: Mol Microbiol. 2016 Apr 1;101(2):194–209. doi: 10.1111/mmi.13362

Table 2.

Down-regulation of clpP1P2 increases susceptibility of M. bovis BCG clpP1-tetoff to ADEP2.

	no ATc	0.05 ng ml⁻¹ ATc	0.1 ng ml⁻¹ ATc	0.2 ng ml⁻¹ ATc	25 ng ml⁻¹ ATc
MIC (μg ml⁻¹) against M. bovis BCG clpP1-tetoff
ADEP2	16 (8)	8 (4)	4 (2)	2 (0.5)	no growth
apramycin	1	1	nd	1	no growth
isoniazid	0.03	0.03	0.03	0.03	no growth

MIC (μg ml⁻¹) against M. bovis BCG wt
ADEP2	16	Nd	nd	nd	16
apramycin	1	Nd	nd	nd	1
isoniazid	0.06	Nd	nd	nd	0.06

MIC values against M. bovis BCG Pasteur wildtype (wt) and M. bovis BCG Pasteur clpP1-tetoff in the presence of increasing ATc concentrations. In this conditional system clpP1P2 expression is suppressed by ATc resulting in decreased ClpP1P2 protein levels (for further information on strain regulation compare figure S5A). For ease of comparison with table 3, data are arranged with decreasing ClpP levels from left to right. Data in brackets represent the concentration where partial growth inhibition was observed. nd, not determined.