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. 2017 Mar 27;37(2):BSR20160257. doi: 10.1042/BSR20160257

Figure 4. Western blot in an ex vivo study (Part 2 experiment).

Figure 4

Western blot analysis of 4E-BP1 and ERK to furthermore confirm the Akt and mTOR on kinase activity in homogenates of the LV from the remote zone in a rat-isolated infarcted heart model. A significantly increased p-4E-BP1 level is noted in the LiCl-treated group compared with that seen in the saline-treated group, which was attenuated after administering deguelin (a specific Akt inhibitor) and rapamycin (an mTORC1 inhibitor). The values are mean ± S.D. (n=5 per group). Experiments were replicated three times and results expressed as the mean value. *P<0.05 compared with saline-, LiCl-deguelin-, and LiCl-rapamycin-treated infarcted rats; †P<0.05 compared with saline-treated infarcted rats.