FIG 2.

SDS-PAGE analysis of Pre-F-GCN4t (A) and Post-F-XC (B) under reducing (with dithiothreitol [+DTT]) and nonreducing (without dithiothreitol [−DTT]) conditions. Lane 1, nonreducing conditions; lane 2, reducing conditions; lane 3, N-glycosidase-treated sample under reducing conditions. Band a, polypeptide with incomplete cleavage at the second furin site; band b, fully processed polypeptide; bands c and f, F1 with pep27; bands d and g, F1 without pep27; bands e and h, F2; band w, higher-order protein structure; M, molecular mass markers. The band at 35 kDa represents the N-glycosidase F enzyme (NG). Expected molecular masses (with and without glycosylations) are shown at the bottom of each gel photograph.