FIG 7.

Effects of apoE and VLDL-containing mouse serum on HCV cell-to-cell spread. HCV-infected Huh-7.5 cells were cocultured with pcDA6/TR-Rluc-transfected NIrD-fluc-sc4 cells at a ratio of 1:4 in the presence of different concentrations (0, 6.25, 12.5, 25, and 50 μg/ml) of apoE-blocking monoclonal antibody mAb23 (A) or various percentages (0%, 2.5%, 5%, and 10%) of mouse serum (B). Normal mouse IgG (mIgG) was used as a control for the apoE monoclonal antibody. After 48 h of coculture, the levels of Renilla and firefly luciferase expression were determined and converted to a percentage of the control in the same way as described in the legends of Fig. 5 and 6.