FIG 2.

Determination of the optimal inoculation route, dosage, and age. Five-day-old ICR mice (n = 10 per group) were i.c., i.m., and i.p. inoculated with different doses of CVA10 strain TA151R (1.0 × 10², 1.5 × 10³, and 2.25 × 10⁴ TCID₅₀/mouse, respectively). Control animals were inoculated with medium. All the mice were monitored daily for clinical symptoms (A to C and K), body weight (D to F and J), and survival rates (G, H, and L) until 14 days postinfection. Mice at 14 and 21 days of age did not develop any significant clinical signs (data not shown). Control animals were administered NS. The data represent the mean values of the results of 10 repeat experiments.