FIG 2.

Vpr does not regulate Env expression in infected MDDCs or incorporation of Env into MDDC-derived virions. (A) Western blot analysis of mock-infected, Lai-YU2 (WT)-infected, or Lai-YU2/ΔVpr-infected MDDCs (MOI = 3) for p55^Gag and gp120 expression at day 6 postinfection. (B) Quantification of Western blots for p55^Gag and gp120 in infected MDDCs, as described above for panel A, from four independent experiments. The gp120 band intensity was quantified and normalized to the p55^Gag values from experiments with infected MDDCs derived from 4 donors. Data shown are means (± standard errors of the means). (C) Western blot analysis of p24^Gag and gp120 expression in mock-infected, Lai-YU2 (WT)-infected, or Lai-YU2/ΔVpr-infected MDDCs (MOI = 5). MDDC culture supernatants were harvested at days 3, 6, and 9 postinfection; pooled; and concentrated over a 20% sucrose cushion, and virus pellets were lysed for Western blot analysis. (D) Quantification of data from Western blot analysis of MDDC-derived virions from three independent donors. The band intensity for gp120 was quantified and normalized to the p24^Gag band intensity. Data shown are means ± (standard errors of the means). Significance was calculated by using a one-sample t test (N.S., not significant [P > 0.05]).