TABLE 3.
Antibodies for immunohistochemical and immunofluorescent antibody staining used in this study
| Cellular phenotype | Antigen | Clone | Assay(s)a | Source and reference(s) |
|---|---|---|---|---|
| T lymphocytes | CD2 | HB88A | IHC | Washington State University (W. Davis); 109 |
| CD3 | LN10 | IHC | Leica Microsystems; 110 | |
| CD3 | UC F6G-3 | IFA | University of California (J. L. Stott); 111 | |
| CD4 | CVS4 | IHC and IFA | AbD Serotec; 112 | |
| CD5 | HT23A | IHC | Washington State University (W. Davis); 113 | |
| CD8 | CVS8 | IHC and IFA | AbD Serotec; 112 | |
| CD25 | 4C9 | IHC | Leica Microsystems; 110 | |
| B lymphocytes | CD21 | B-ly4 | IHC and IFA | BD Pharmingen; 40 |
| Tissue macrophages and dendritic cells | CD18 | CA18.2G1 | IHC | University of California (P. F. Moore); 45 |
| CD83 | HB15E | IHC | BD Pharmingen; 114 | |
| CD86 | IT2.2 | IHC | BioLegend; 114 | |
| CD163 | AM-3K | IHC | Transgenic; 115 | |
| CD172a | DH59B | IHC | Washington State University (W. Davis); 16, 71 | |
| CD204 | SRA-E5 | IHC | Transgenic; 116 | |
| Iba-1 | NAb | IHC | Wako Chemicals; 48 | |
| MHC-II | CR3/43 | IHC | Dako; 117 | |
| Calprotectin/L1 | MAC387 | IHC | AbD Serotec; 56 | |
| Epithelial and mesenchymal cells | Pan-cytokeratin | AE1/AE3 | IFA | eBioscience; 118 |
| Vimentin | V9 | IFA | eBioscience; 119 |
a
IHC, immunohistochemistry; IFA, immunofluorescent antibody staining.
b
NA, not applicable.