FIG 7.

Deletion of the A52R, B15R, and K7R genes affects humoral Env-specific IgG immune responses. Shown are vaccine-induced HIV-specific IgG responses in mice (n = 4/group) immunized following a heterologous DNA prime/virus boost regimen (10⁷ PFU of NYVAC-C or NYVAC-C deletion mutants). (A to C) Levels of Env-specific total IgG (A), IgG1 (B), and IgG3 (C) antibodies in serum of mice (n = 4/group) 11 days postinfection with NYVAC-C or NYVAC-C deletion mutants. Data are represented as optical density (OD) in arbitrary units (AU). Nonspecific responses of mice infected with control NYVAC-WT were subtracted from total values. Data are representative of two independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001.