FIG 3.

Seroconversion and latency establishment after intranasal inoculation of gp150⁺ and gp150⁻ MuHV-4. Female mice were infected intranasally with WT or gp150⁻ MuHV-4 (10⁴ PFU under general anesthesia). At 1 month p.i., sera and spleen were collected. (A) ELISA of sera shows no difference in MuHV-4-specific antibodies between groups (P > 0.05 by one-way analysis of variance and a Bonferroni post hoc test). OD 405, optical density at 405 nm. (B and C) Viral genome copy numbers in spleen measured by qPCR show no significant difference between groups (P > 0.05 by one-way analysis of variance and a Bonferroni post hoc test) (B), nor did infectious center assays of spleens show a difference between groups (P > 0.05 by the Student test) (C). Mean values are represented by histograms.