FIG 2.

LIMK inhibitors discovered from anti-HIV screening. Rev-CEM-GFP-Luc HIV Rev-dependent indicator T cells were pretreated with LIMK inhibitors (100 μM) or DMSO for 1 h and then infected with HIV-1(NL4-3) for 3 h. The cells were washed to remove the virus and the inhibitors and incubated for 48 h. HIV-dependent GFP expression was measured by flow cytometry. PI was added during flow cytometry to simultaneously measure cell viability. Only viable cells (gated R1) were used for GFP quantification. The percentages of GFP⁺ cells are shown.