Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun 13;8:1082. doi: 10.3389/fmicb.2017.01082

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 Miyazaki, Miyake, Doi, Koma, Uchiyama, Adachi and Nomaguchi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Comparative analysis of several CA proteins for their in vitro polymerization properties. CA-polymerization was performed in vitro and monitored by OD at 350 nm as described in MATERIALS AND METHODS. (A) Polymerization of NL4-3 CA (NLCA), GL-AN CA (GLCA), and NL/GL CA (NL/GL) proteins for 4 h at various NaCl concentrations. The chimeric clone NL/GL has the sequence encoding the NTD of NL4-3 CA and the linker domain/CTD of GL-AN CA (Figure 1A). (B) Polymerization kinetics of NL and NL/GL CA proteins (1.5 M NaCl). (C) Polymerization of NL and GL32NL CA proteins for 4 h at various NaCl concentrations. GL32NL is a chimeric NLCA-derivative clone which has the sequence encoding the very N-terminal region of GL-AN CA (Pro1-Phe32 in Figure 1A).