FIG. 7.

(A) Immunofluorescence analysis of intracellular schizonts indicated that antibodies against the inner membrane complex protein IMC3 do not label distinct structures until late in development. (B) Covisualization of IMC3 and SnSAG3 revealed significant association between the inner membrane complex and the surface antigen. Host cells infected with S. neurona were fixed with methanol on day 4 postinfection. Slides were labeled with rabbit anti-IMC3 and rat anti-SnSAG3, followed by Texas Red-conjugated anti-rabbit IgG and FITC-conjugated anti-rat IgG. Bars = 5 μm.