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. 2017 Jun 7;37(23):5620–5633. doi: 10.1523/JNEUROSCI.2617-16.2017

Figure 2.

Figure 2.

Netrin-1 reduces the subcellular overlap of UNC5C with TUBB3 in the GC of primary neurons. A–C, Overlap of immunofluorescent signals of UNC5C (A) and TUBB3 (B) in the GC of mouse EGL cells. P2 mouse EGL neurons were cultured for 20 h and stimulated with a sham-purified control. C, Merged image of A, B. A–C, The value of PCC is 0.59 ± 0.02. D–I, Overlap of endogenous UNC5C with TUBB3 in the GC of EGL neurons was reduced after netrin-1 stimulation for either 5 min (D–F) or 10 min (G–I). F, I, Merged images of D, E and G, H, respectively. D–F and G–I, The value of PCC of UNC5C and TUBB3 is 0.49 ± 0.02 and 0.37 ± 0.02, respectively. Scale bar, 10 μm. J, Quantitative analysis of PCC in the control and netrin-1 groups. A total of 37 GCs in each group were analyzed. ***p < 0.001 (one-way ANOVA and Tukey's test for post hoc comparisons). K, Quantification of PCC after 90 degree counterclockwise rotation of one fluorescence channel. ***p < 0.001 (one-way ANOVA and Tukey's test for post hoc comparisons). R, Rotation.