Figure 4.

TUBB3 is required for netrin-1/UNC5C-mediated axon repulsion. A, Schematic diagram of axon turning of P2 mouse EGL cells in a control (left) or netrin-1 (right) gradient using a Dunn chamber axon guidance assay. B, Live-cell imaging showing axon turning of P2 EGL cells transfected with Venus-YFP only or combination of Venus-YFP with other indicated constructs. Nucleofection of Venus-YFP into P2 EGL neurons allowed visualization of axon projection. A left-to-right netrin-1 gradient was established as shown in A. Scale bar, 10 μm. C, Quantification of axon turning of P2 EGL neurons. Data are mean ± SEM. ***p < 0.001 (one-way ANOVA and Tukey's test for post hoc comparisons). The numbers on the top of each bar indicate the numbers of GCs analyzed in the corresponding groups.