Figure 1.

Generation of a Kv2.1-derived, STX1A-binding peptide sequence. A, Far-Western assay of the proximal Kv2.1 C-terminus (C1a) region using 15 aa segments spanning residues Kv2.1 451–540, in overlapping 1 aa steps. Two peptides, highlighted in blue and red, flanked a region of high STX1A binding. Error bars indicate mean ± SEM of signal intensity in four independent assays in the absence and presence (black bars) of 100 μm of the derived TAT-containing STX1A-binding peptide described in C below. B, Representative peptide spot-array of the far-Western experiment. C, Final sequences of the peptides used in this study are shown. Orange sequence represents the cell-permeable HIV transactivator of transcription domain (TAT). Red sequence represents the STX1A-binding domain derived from Kv2.1. Green sequence represents a scrambled control based on the C1aB sequence.