Figure 1.
CFAP69 is an evolutionarily conserved protein enriched in OSN cilia. A, Schematic of the CFAP69 protein. CFAP69 consists of 942 aa and is predicted to have ARM repeat domains. B, Evolutionary relationships of taxa based on protein sequences. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances. The evolutionary distances are in the units of the number of amino acid substitutions per site. The sum of branch length in the tree is 4.53259594. C, X-gal staining of OE sections from control and Cfap69tm1a mice. LacZ is expressed in the OE in Cfap69tm1a mice (right), but not in controls lacking the tm1a allele (left). Scale bar, 20 μm. D, Western blot analysis of OE and olfactory cilia preparations for CFAP69. Lanes were loaded with the same amount of total proteins. E, Immunofluorescent staining showing that CFAP69 is expressed in the cilial layer of the OE and colocalizes with acetylated tubulin, a marker of the cilia. Scale bar, 20 μm.