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. 2017 Mar 27;37(2):BSR20160445. doi: 10.1042/BSR20160445

Figure 3. PKC β inhibitor enhances the cisplatin-induced autophagy in cervical cancer Hela cells.

Figure 3

Hela cells were transfected with EGFP–LC3 II reporter plasmid for 6 h and were treated with 0 or 20 μM cisplatin and with 0, 1 or 3 μM enzastaurin for 24 h, then Hela cells were observed under fluorescence microscopy. The image (A) and the number (B) of autophagic puncta were presented/counted respectively. And the autophagy-associated markers, such as microtubule-associated protein 1 light chain 3 (LC3)-I/II and autophagy protein 5 (ATG 5) were analysed by Western blotting assay (C) and were presented as ratio (D) of LC3-II/I and Atg 5 to GAPDH. Experiments were performed independently in triplicate; ns, no significance; * P<0.05, **P<0.01, *** P<0.001 or ****P<0.0001.