FIG. 5.

E. intestinalis spore adherence to host cells with reduced sulfated surface proteoglycans by sodium chlorate treatment. Vero host cells grown on glass coverslips in 12-well plates were treated with 10, 20, 40, or 60 mM sodium chlorate or control sodium chloride for 24 or 48 h prior to the standard spore adherence assay. The percentage of attached spores relative to spore adherence on nontreated host cells is shown. The values for all sodium chlorate-treated samples are significantly different from those for either sodium chloride-treated or nontreated controls (P < 0.0001). The data presented are from one experiment of three that were performed with similar results.