Table 3. Infection rates, transmission rates and median titers of Ae. aegypti mosquitoes intrathoracically injected with ZIKV, CHIKV, or both ZIKV and CHIKV.

Infection and transmission rates of mosquitoes were determined as the percentage of mosquitoes with either ZIKV, CHIKV, or both viruses in their body or saliva, respectively, out of the total number of injected mosquitoes within the respective treatment. Infection and transmission rates are presented as percentages (number of virus positive mosquito bodies or saliva samples / total number of engorged mosquitoes). Titers were determined for mosquitoes with a fully disseminated infection of ZIKV, CHIKV, or both. The results represent the cumulative data from three independent biological replicates.

Infection	Virus	Dose (TCID50/ml)	Infection rate (%)	Transmission rate (%)	Median titer body (TCID50/ml)	Median titer saliva (TCID50/ml)
Single-infection	ZIKVSUR	2.8 × 103	100 (49/49)	77.6 (38/49)	2.0 × 107	1.0 × 103
	CHIKV37997	2.8 × 103	100 (48/48)	22.9 (11/48)	2.0 × 106	1.0 × 103
Co-infection	ZIKVSUR	2.8 × 103	100 (48/48)	68.8 (33/48)	5.4 × 107	4.2 × 103
	CHIKV37997	2.8 × 103	100 (48/48)	27.1 (13/48)	6.3 × 106	1.0 × 103
	ZIKVSUR & CHIKV37997		100 (48/48)	20.8 (10/48)