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. 2017 Jan 10;16:1536012116685941. doi: 10.1177/1536012116685941

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© The Author(s) 2017

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 6. — Representative TUNEL-stained images of tumor specimen samples from different time points: (A) baseline, (B) 1 day after 1 dose of 10 mg/kg doxorubicin treatment, and (C) 3 days after a second dose of doxorubicin treatment in the UM-22B group. Apoptotic nuclei are shown in green. Normal cell nuclei are shown in blue, stained by DAPI. (D) TUNEL-positive cells counted and these counts were used to determine the percentage of apoptotic cells in the field. This was repeated for 3 fields of view for the 1- and 3-day groups, and on 2 fields for the day 0 group. DAPI indicates 4′,6-diamidino-2-phenylindole.