TABLE 2.

Oligonucleotides used and PCR product sizes

Primer name	Oligonucleotide sequence (5′-3′)	PCR product size (bp)	Use
IM htrA-1	TCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACAAAGCCACGTTGTGTCTCAA	1,057	LF82-ΔhtrA isogenic mutant construction
IM htrA-2	CAGATTGTAAGGAGAACCCCTTCCCGTTTTCAGGAAGGGGTTGAGGGAGATTAGAAAAACTCATCGAGCA
htrA-1 htrA-2	GCGAACTGATCGGTATCAAC GAGTTCAGCTCAGTCCCCA	168	Allelic replacement of htrA in LF82-ΔhtrA and RT-PCR
htrA-3 htrA-4	GACCGACCTCTATGCGTGGG GTGCCACCATATCCTGCGCC	1,812	Allelic replacement of htrA in LF82-ΔhtrA
BamHI-htrA	CGGGATCCCGCCAGGCTTTTGTAAAGACG	170	Promoter htrA-lacZ fusion construction
EcoRI-htrA	GGAATTCCTGCTAATGTGGTTTTTTTCATGT
IM cpxR-1	CGCCAGCGTCAGCCAGAAGATGGCGAAGATGCGCGCGGTTAAGCTGCCTATTAGAAAAACTCATCGAGCA	1,057	LF82-ΔcpxR isogenic mutant construction
IM cpxR-2	GGATTAGCGACGTCTGATGACGTAATTTCTGCCTCGGAGGTATTTAAACAAAAGCCACGTTGTGTCTCAA
cpxR-1 cpxR-2	GCGCAGATGACTATCTCCCG GTTTGCCCGTCGAAGCTGGC	187	Allelic replacement of cpxR in LF82-ΔcpxR
cpxR-3 cpxR-4	CGGCAGCGGTAACTATGCGC TGGCGTGAATCGAGCTTGGG	969	Allelic replacement of cpxR in LF82-ΔcpxR
IM rpoE-1	TTTGTTATCAATAGCTTCCCTCGCTCGGAAGATACGTGAACGCACCGTACTTAGAAAAACTCATCGAGCA	1,057	LF82-ΔrpoE isogenic mutant construction
IM rpoE-2	TGGCGTTTCGATAGCGCGTGGAAATTTGGTTTGGGGAGACTTTACCTCGGAAAGCCACGTTGTGTCTCAA
A2 GBL-3 B2 GBLnp-5	AAAGCCACGTTGTGTCTCAA TTAGAAAAACTCATCGAGCA	957	Kanamycin resistance cassette amplification