TABLE 3.
Abilities of LF82 Tn5phoA mutants to replicate within J774-A1 macrophages at 24 h postinfection
| Strain or mutant | Tn5phoA insertion site | CFU per well (105)a
|
% of intracellular bacteria at 24 h postinfectionb | |
|---|---|---|---|---|
| 1 h postinfection | 24 h postinfection | |||
| K-12 | 0.254 ± 0.025 | 0.006 ± 0.005 | 2.1 ± 1.8 | |
| LF82 | 8.1 ± 3.5 | 17.3 ± 1.6 | 319.5 ± 110.1 | |
| 2E12 | dsbA | 8.3 ± 2.0 | 0.9 ± 0.4 | 9.2 ± 2.5 |
| 3G1 | 11.2 ± 3.6 | 1.9 ± 0.6 | 18.2 ± 2.5 | |
| B9D | 13.0 ± 4.6 | 1.6 ± 0.7 | 11.3 ± 2.4 | |
| OA8 | yfgL | 4.3 ± 1.7 | 1.6 ± 0.9 | 30.9 ± 6.2 |
| 51F8 | 3.4 ± 1.1 | 0.9 ± 0.3 | 28.6 ± 8.6 | |
| 52E8 | htrA | 8.3 ± 1.9 | 2.8 ± 0.9 | 32.3 ± 5.8 |
| 54H6 | 11.8 ± 3.9 | 4.2 ± 2.2 | 34.1 ± 8.2 | |
| 55H8 | 9.3 ± 3.4 | 1.3 ± 0.4 | 15.1 ± 1.1 | |
| 56A6 | 7.6 ± 1.9 | 1.5 ± 0.5 | 14.6 ± 4.5 | |
| 57G3 | 10.1 ± 2.4 | 2.7 ± 1.0 | 26.3 ± 7.0 | |
| 6H3 | slyB | 13.3 ± 5.3 | 1.6 ± 0.9 | 9.5 ± 2.8 |
| 53H2 | yraP | 11.5 ± 4.1 | 4.1 ± 1.7 | 44.9 ± 14.8 |
a
Mean number of bacteria surviving gentamicin treatment per well ± standard error of the mean (SEM).
b
Mean percentage ± SEM of intracellular bacteria at 24 h postinfection relative to the number of intracellular bacteria at 1 h postinfection, taken as 100%.