TABLE 2.
Protein cytokine levels in B-cell-deficient and C57BL/6J mice 14 days after infection with C. neoformansa
| Cytokine | Mouse background | Mean cytokine level ± SD (pg/ml) in mice treated with:
|
|||
|---|---|---|---|---|---|
| CN/PBSb | CN/IgG1b | PBS/PBS | PBS/IgG1 | ||
| IL-2 | μMT−/− | 708.0 ± 290.5 | 730.3 ± 45.5 | 941.3 ± 52.0 | 1,100.8 ± 14.4 |
| C57BL/6J | 956.2 ± 196.7 | 919.4 ± 102.8 | 949.8 ± 39.9 | 807.8 ± 130.5 | |
| IL-4 | μMT−/− | 823.4 ± 283.2c | 1,081.3 ± 240.1 | 1,129.2 ± 29.3 | 1,341.0 ± 8.06 |
| C57BL/6J | 1,493.9 ± 173.9c,d | 1,505.5 ± 113.8 | 985.4 ± 7.92 | 884.4 ± 161.4d | |
| IL-6 | μMT−/− | 1,113.4 ± 390.1 | 1,356.7 ± 292.3 | 1,051.0 ± 80.6 | 1,004.8 ± 59.2 |
| C57BL/6J | 988.3 ± 144.9d | 1,169.4 ± 129.9d | 705.9 ± 106.1d | 550.5 ± 206.4d | |
| IL-10 | μMT−/− | 1,429.2 ± 372.6e,f | 1,732.1 ± 358.2 | 2,355.3 ± 69.9f | 2,135.4 ± 88.9 |
| C57BL/6J | 1,897.4 ± 162.7e,g | 2,210.8 ± 286.2g | 2,104.8 ± 126.6 | 1,737.6 ± 585.2 | |
| IL-12 | μMT−/− | 2,870.5 ± 1,124.4h | 3,064.2 ± 380.0f | 3,552.5 ± 148.5 | 4,762.5 ± 116.7h |
| C57BL/6J | 4,387.5 ± 1,355.1j | 5,732.3 ± 1,449.9i | 3,340.0 ± 186.7 | 2,736.0 ± 690.1j | |
| TNF-α | μMT−/− | 833.8 ± 495.1 | 1,082.8 ± 31.6 | 1,091.7 ± 15.1 | 1,190.5 ± 163.4 |
| C57BL/6J | 934.0 ± 204.5 | 1,044.8 ± 56.4 | 1,020.7 ± 24.5 | 1,022.7 ± 163.1 | |
μMT−/− and C57BL/6J mice received 1 mg of IgG1 or PBS 24 h prior to infection with 106 C. neoformans cells. Mice were killed 14 days later. Statistical analysis was performed with the Student t test and one-way ANOVA. Six mice were in each group except the sham-infected groups, for which three mice were used.
Sham-infected mice were given PBS at the time of infection.
Compared with values for C57BL/6J control-treated mice, P was < 0.001.
Compared with values for C57BL/6J sham-infected mice, P was < 0.05.
Compared with values for μMT−/− control-treated mice, P was equal to 0.02.
Compared with values for μMT−/− sham-infected mice, P was 0.02.
Compared with values for C57BL/6J MAb 2H1-treated mice, P was 0.056.
Compared with values for μMT−/− sham-infected mice, P was 0.01.
Compared with values for C57BL/6J MAb 2H1-treated mice, P was 0.03.
Compared with values for C57BL/6J sham-infected mice, P was 0.056.