FIGURE 5:

Podocyte IRE1α deletion induces ER stress and causes deficient autophagosome turnover in 4- to 5-mo-old prealbuminuric mice. (A, B) Glomerular lysates were immunoblotted with antibodies as indicated. Representative immunoblots of four IRE1α^flox/flox;+ (M +) and four IRE1α^{flox/flox;Cre} (M Cre) mice obtained from a cohort of prealbuminuric male mice. (C, D) Densitometric quantification. M Cre mice show elevated expression of Grp78 (*p = 0.033) and Grp94 (*p = 0.0017). No change in calnexin was noted (B). (B, H) M Cre mice display elevated global polyubiquitinated proteins (*p = 0.0015). (A, E, F) M Cre mice show decreased LC3B-II, normalized either to LC3B-I (*p = 0.023) or actin (*p = 0.043; five to seven mice per group); M Cre mice show a reduced LC3B-II/I ratio (*p = 0.002). There were no differences in total LC3B-I or phospho-JNK1 (pJNK1) between groups (F, G).