FIGURE 6:
Mice with podocyte IRE1α deletion are deficient in autophagy but do not show ER stress at 9 mo of age. (A) Glomerular lysates were immunoblotted with antibodies as indicated. Representative immunoblots of 5 IRE1αflox/flox;+ (M +) and 5 IRE1αflox/flox;Cre (M Cre) mice. (B–D) Densitometric quantification. M Cre mice show decreased LC3B-II, normalized either to LC3B-I (*p = 0.013) or actin (*p < 0.0001), and decreased JNK1 phosphorylation (*p = 0.048). (E–I) No significant differences between M Cre and M + mice were observed in Grp78, Grp94, podocalyxin, nephrin maturation (upper/lower band), or mature nephrin expression (upper band).