FIGURE 1:
Ectopically expressed myc7-Glut4 is colocalized with LysoTracker in undifferentiated (Fb) but not in differentiated (Ad) 3T3-L1 cells. 3T3-L1 cells stably expressing myc7-Glut4 were incubated with LysoTracker, fixed, and stained with monoclonal anti-myc antibody and FITC-conjugated donkey anti-mouse IgG and analyzed by double immunofluorescence.