FIGURE 6:

Knockout of Vps35 in wild-type 3T3-L1 adipocytes decreases stability of sortilin and Glut4 in a bafilomycin A–sensitive manner. (A) total lysates (80 µg) of wild-type and CRISPR/Cas9-edited 3T3-L1 adipocytes were analyzed by Western blotting. (B) Half-life of sortilin and Glut4 was measured in wild-type and CRISPR/Cas9-edited 3T3-L1 adipocytes in the absence and presence of bafilomycin A as described in Materials and Methods. A representative result of three interdependent experiments. (C) Quantification of data shown in B. Each data point represents normalized mean value ± SE of three experiments.