FIGURE 7:

Knockout of Vps35 in 3T3-L1 adipocytes inhibits insulin-stimulated glucose uptake but not insulin signaling. (A) Total lysates (80 µg) of wild-type and CRISPR/Cas9-edited 3T3-L1 adipocytes treated and not treated with 100 nM insulin for 15 min were analyzed by Western blotting. (B) Insulin-stimulated glucose uptake was measured in wild-type and CRISPR/Cas9- edited 3T3-L1 adipocytes as described in Materials and Methods. **p < 0.01; NS, not significant. Results were analyzed by Student’s t test. A representative result of three independent experiments.