TABLE 1:

Quantitative analysis of protein colocalization.

Line	Cell type	Transfected construct(s)	Staining 1	Staining 2	Corresponding figure	Pearson’s R
1	Preadipocyte	myc7-Glut4	myc	LysoTracker	Figure 1 (top)	0.88 ± 0.04
2	Adipocyte	myc7-Glut4	myc	LysoTracker	Figure 1 (bottom)	0.48 ± 0.09
3	Preadipocyte	myc7-Glut4	myc	Vps35	Figure 2B (top)	0.28 ± 0.13
4	Adipocyte	myc7-Glut4	myc	Vps35	Figure 2B (middle)	0.76 ± 0.08
5	Adipocyte	myc7-Glut4	myc	Sortilin	Figure 4A	0.80 ± 0.08
6	Adipocyte	myc7-Glut4	myc	LysoTracker	Figure 3B (bottom)	0.86 ± 0.06
		Sortilin shRNA
7	Adipocyte	myc7-Glut4	myc	Vps35	Figure 3B (top)	0.50 ± 0.08
		Sortilin shRNA
8	Preadipocyte	myc7-Glut4	Glut4	LysoTracker	Figure 5 (bottom)	0.43 ± 0.08
		Sortilin-myc/His
9	Preadipocyte	myc7-Glut4	Glut4	Vps35	Figure 5 (top)	0.67 ± 0.10
		Sortilin-myc/His
10	Adipocyte	None	Glut4	LysoTracker	Figure 8C (top)	0.52 ± 0.11
11	Adipocyte	None	Glut4	Vps35	Figure 2B (bottom)	0.82 ± 0.09
12	Adipocyte	Vps35 CRISPR	Glut4	LysoTracker	Figure 8C (bottom)	0.82 ± 0.06

Pearson’s R was determined by ImageJ analysis of 12–14 randomly selected cells.